EpiAlveolar tissues are produced on cell culture inserts by differentiating primary alveolar epithelial cells, 肺内皮细胞和成纤维细胞 using a series of proprietary media. Differentiated EpiAlveolar tissues have a squamous epithelium consisting of Type I and Type II alveolar epithelial cells cultured with extracellular matrix producing pulmonary fibroblasts and endothelial cells. The epithelium exhibits functional tight junctions and a robust barrier that remains stable for studies lasting more than 30 days.
A part of the funding for the development of EpiAlveolar was provided by the 善待动物组织国际科学联盟.
The EpiAlveolar 3D human tissue model is routinely utilized for a range of applications including safety & 风险评估、炎症 & 纤维化和药物输送. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, 不是几周或几个月.
Use EpiAlveolar to determine relative safety and toxicological potential of inhaled substances.
Hana Barosova, Anna G. Maione, Dedy Septiadi, Monita Sharma, 莱提纱Haeni, Sandor Balog, 奥利维亚·奥康奈尔, George R. Jackson, David Brown, Amy J. Clippinger, 帕特里克·海登, Alke Petri-Fink, Vicki Stone, and 芭芭拉Rothen-Rutishauser. ACS Nano Article ASAP. DOI: 10.1021/acsnano.9b06860
Kit: A standard EpiAlveolar kit (ALV-100-FT-PE12) consists of 24 tissues. (纸巾“包”包含纸巾, 250 mL培养基, and plasticware; contact 正规的网博软件有哪些 for specific kit contents). ALV-100-FT-MAC-PE12, containing THP-1 macrophages is also available.
Formats: 12mm Transwell inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
Lot numbers: 每周生产的纸巾批次都有一个特定的批号. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有组织试剂盒在细胞方面都是相同的, medium, handling, 培养条件, etc.
Shipment Day: ALV-100-FT-PE12 -每周一. ALV-100-FT-MAC-PE12 -每周二.
Delivery: ALV-100-FT-PE12 -星期二上午经联邦快递优先服务(美国). 美国以外:周二至周三，视地点而定. ALV-100-FT-MAC-PE12 – Wednesday morning via FedEx priority service (US). 美国以外:周三至周四，视地点而定.
Shelf Life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. 但是，不建议延长储存时间. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day.
实验时长: Tissue cultures can be continued for TWO (2) WEEKS or more with good retention of normal morphology. 纸巾必须每隔一天喂5次.0 ml维护培养基(ALV-100-MM). Cell culture inserts are placed into 6-well hanging culture plates provided (HNG-TOP-6-22).
Type: Normal human alveolar epithelial cells (NHAE); Normal human pulmonary fibroblasts (NHPF), Normal human pulmonary endothelial cells (NHPE) and THP-1 macrophages (ALV-100-FT-MAC-PE12).
Derived from: 身体健康,不吸烟
Screened for: HIV，乙型肝炎，丙型肝炎，支原体
Base medium: Proprietary
抗真菌剂: 两性霉素B 15ng /ml
pH Indicator: Phenol red
目视检查: All tissues are visually inspected and if physical imperfections are noted, 纸巾被拒绝装运
最终用途测试: Transepithelial electrical resistance (TEER) of each EpiAlveolar lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments).
Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
病原体筛查: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen-free. Thus, these products and all human-derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, 微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 正规的网博软件有哪些 technical service
不合格批件通知: 如果纸巾没有通过我们的质量检验或无菌检验, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌检测是在出货后完成的, a notification will be made as soon as possible (Under normal circumstances, TEER故障将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)